Identification and anti-inflammatory activity

of homarine isolated from Anemonia sulcata’s tentacles

 

H. Aassila1*, M. Guyot2, A. El Abbouyi1, A. Fassouane1

1 Laboratoire de Biochimie, Faculté des Sciences, Université Chouaib Doukkali, BP 20,  24000 El Jadida, Maroc.

2Unité MCAM, UMR 7245 CNRS/MNHN, Dept. RDDM. Case postale 54 (63 rue Buffon)  57 rue Cuvier, 75005 Paris France

* Corresponding author. E-mail: aahinde@hotmail.fr

Received: 15 April 2013; revised version accepted: 04 July 2013

 

Abstract

     An anti-inflammatory agent, produced by Anemonia sulcata originating from Atlantic coast of El Jadida city (Morocco), was isolated and characterized. It was extracted from Anemonia sulcata’s tentacles by 80/20 (V/V) dichloromethane / ethanol and purified by gel filtration and silica gel chromatography. Its chemical structure was assigned to 2-carboxy-1-methylpyridinium by analysing 1D (1H, 13C), 2D(HMBC, HMQC, COSY) NMR and mass spectral data.

     Homarine showed an excellent and new anti-inflammatory activity assessed by two different models: Enzymatic model (Phospholipase A2 obtained from bee venom) and cellular model (superoxide anions generation by Rat pleural polymorphonuclear leukocytes).

 


Keywords: Anti-inflammatory activity; Homarine; Anemonia sulcata's tentacles.

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