Isolation of Anticancer Natural Ingredients

from Sour Orange


H. M. Odeh1*, M.A. Al-Nuri2, B. Hammouti3, T. Ben Hadda4, I. Warad2*

1 Department of Chemical Engineering, AN-Najah National University, P. O. Box 7, Nablus, State of Palestine.

2 Department of Chemistry, AN-Najah National University, P. O. Box 7, Nablus, State of Palestine.

3LCAE-URAC18, Faculty of Sciences, University Mohammed Ier, Oujda-60000, Morocco.

4Laboratoire LCM, Faculty of Sciences, University Mohammed 1er, Oujda-60000, Morocco.

Received 2014, Revised 2014, Accepted 2014

* Corresponding author. E-mail: (H.M. Odeh), (I. Warad)

Received: 03 December 2013; revised version accepted: 18 January 2014



     Potential anticancer constituents of sour orange (Citrus aurantium L.) were isolated from ethyl acetate extract of sour orange.  The extraction process of limonoid aglycones from sour orange seeds and peels was investigated using different solvents. Continuous Soxhlet extraction method with solvents having increasing polarities such as n-hexane, ethyl acetate, methanol and methanol/water were used.  These extract fractions were processed on silica-gel column and silica-gel plate chromatography. The purity of the isolated compounds was analyzed by TLC and HPLC. Finally, UV active components were isolated and identified. The degree of isolation is followed up by thin layer chromatography (TLC) and high pressure liquid chromatography (HPLC). The identification of limonoid compounds were based on pure limonid standard. The isolated compounds were tested for the inhibition of human prostate cancer cells. The experiments were conducted at genetic laboratory in An-Najah University. Cell proliferation, arrest of cell growth, and induction of apoptosis were determined by MTT assay.


Keywords: Anticancer; Citrus aurantium L; Limonoid.

© 2015