Modulation of separation selectivity of inorganic anions in ion exchange chromatography with evaporative light scattering detector by the use of unmodified polymer-based anion exchangers

M. El Haddad1,2, R. Boulahjar2,3, S. El Antri3, M. Akssira2, M. Dreux4, S. Lazar3,*

1Faculté Poly disciplinaire, Université Cadi Ayyad, BP 4162, 46000 Safi, Morocco

2Laboratoire de Chimie Bioorganique & Analytique, 3Laboratoire de Biochimie, Environnement & Agroalimentaire

Université Hassan II-Mohammedia, FST, BP 146, 20 800, Mohammedia, Morocco

4Institut de Chimie Organique & Analytique, Université d’Orléans, UMR 6005,

BP 6759, 45 067 Cedex 2, Orléans, France

* Corresponding author. E-mail: lazar_said@yahoo.fr

Received: 22 March 2005; revised version accepted: 20 September 2005

Abstract

Selectivity and retention of inorganic anions (chloride, nitrate, phosphate and sulfate) were studied on unmodified polymer-based anion exchangers; PRP-X100, Metrosep anion dual1, under isocratic elution conditions by ion exchange chromatography followed by evaporative light scattering detector. Sublimate salts; ammonium formate (HCOOH/NH3) and a mixture of formic acid and hydrogen carbonate ammonium (HCOOH/HCO3NH4) and two evaporable perfluorinated acids; trifluoroacetic acid (CF3COOH) and heptafluorobutyric acid (C3F7COOH) with different concentrations are evaluated as ions developers and are compatible with volatility requirements of evaporative light scattering detector. Selectivity and retention of inorganic anions have been modulated with different eluents concentrations and several separations have been investigated on these anionic exchangers. The proposed methods provide performances for analysis of inorganic anions in low-moderate to high ionic strength standard mixture.

Keywords: Ion exchange chromatography; Inorganic anions; Unmodified polymer-based columns; Separation selectivity.

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