Determination of atractyloside
from Atractylis gummifera L. by HPLC method
N. Zaim1, L. Guemouri1, N. El Abbadi2, E. Fouzi3, A. Benjouad4, M. El Mzibri2*
1 Université Hassan II Aïn Chock - Faculté de Médecine Dentaire – Casablanca, Maroc
2 Centre National de l’énergie des sciences et des techniques nucléaires – Rabat, Maroc
3 Université Mohamed V - Faculté de Médecine et de Pharmacie – Rabat, Maroc
4 Université Mohamed V - Faculté des Sciences – Rabat, Maroc
* Corresponding author. E-mail: firstname.lastname@example.org
Received: 10 March 2007; revised version accepted:30 May 2007
Atractyloside (ATR) is a diterpenoid glycoside that occurs naturally in various plants. Consumption of plants containing ATR has caused fatal intoxications. A high performance liquid chromatography (HPLC) method was used for the identification and determination of ATR. The analysis was performed on C8 column utilising a gradient elution profile. The eluant was water-acetonitrile gradient, in the presence of trifluoroacetic acid (0.05%), and at a flow rate of 1.0 ml/min and UV detection at 210 nm. The method showed to be linear (r2 > 0.99) and precise (R.S.D < 5 %). The detection and quantification limits were 2µg/ml and 12.5µg/ml respectively. This method was used in the quantification of various aqueous extracts of Atractylis gummifera L. roots. ATR concentration was found between 2.9 and 4.3 mg/ml according to the region and the season of harvest.
Keywords : Atractylis gummifera L; Atractyloside; HPLC.