Extraction of gelatinous proteins from untanned wastes, their concentration using membrane filtration

and their characterisation

 

A. Gammoun1,3, S. Tahiri2*, N. Saffaj3, H. Loukili3, A. Albizane3,  

S. Alami Younssi3, M. Azzi1, M. de la Guardia4

1 Faculté des Sciences. Université Hassan II – Aïn Chock. Casablanca. Maroc. (agammoun@yahoo.fr)

2 Faculté des Sciences d’El Jadida. Université Chouaïb Doukkali. B.P.20. El Jadida. Maroc.

3 Faculté des Sciences et Techniques. Université Hassan II - Mohammedia. Maroc.

4 Departamento de Quimica Analitica - Edificio de Investigacion. Universidad de Valencia.

C/Dr.Moliner, 50 - 46100 Burjassot. Valencia. España.

* Corresponding author. E-mail: stahiri2000@yahoo.fr

Received: 29 September 2006; revised version accepted: 11 January 2007

 

Abstract

    Gelatinous proteins were extracted from untanned solid wastes generated by tannery after elimination of hair and greases with chemical treatment. Membrane ultrafiltration was studied to concentrate the extract of soluble gelatine. Characteristics of gelatinous products as concentration of amino acids, elemental composition, inorganic anions and nitrogen amount were determined to give a good estimation of protein quality. Results show clearly that recovered material consists mainly of proteins (~ 91.9 %) with very interesting composition of amino acids. The amount of chloride, nitrate and sulphate in recovered gelatine is 3, 0.8 and 1.5 % (w/w), respectively. ICP analysis shows that the concentration of elements is low. Thus, the process used in this work is very efficient for recovery of gelatinous proteins with high purity. Amino acids composition determination was compared with that of some commercial proteinaceous products.

 

Keywords: Untanned wastes; Gelatinous proteins; Membrane; Characterization.

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