Development in vitro model to study the inhibiting effect of some substances on oxalocalcic crystallization

M. Touhami, A. Oussama*, M. Berkani F. Kzaiber

Laboratoire de Spectrochimie Appliquée et Environnement, Unité d’urolithiase,

Faculté des Sciences et Techniques, Université CADI-AYYAD, Beni-Mellal, Maroc

* Corresponding author. E-mail:

Received : 05 April 2004; revised version accepted : 10 September 2004


To include and understand the phenomenon of urinary lithiasis formation and especially the principal identified component: calcium oxalate , several methods were required [1, 2, 3, 4]. For this objective, we carried out in our laboratory a local model to follow in vitro a calcium oxalate crystallization, in supersaturated aqueous solutions, with water circulation in a closed system at 37°C. The principal advantages of this method are a good reproducibility and the possibility of following in continuous different stages of calcic salts crystallization. We applied this model to the comparative study of the inhibiting capacity of three juices: lemon, orange and pomegranate. The results show that the lemon and orange juices present an inhibiting capacity on the crystalline growth of calcium oxalate, and the inhibiting is more remarkable in the case of juice lemon. However, the juice of the grenade presents a contrary effect. These results were confirmed with polarized light microscopy in various time intervals. The microscopic observation we showed the presence of various types of calcium oxalate crystals: monohydrated (COM), dehydrated (COD) and trihydrated (COT) with different frequencies and sizes which vary during of the reaction time. Several works will be carried out in order to determine, separately the activity of these substances as well in-vitro as in- vivo.

Keywords: Urinary lithiasis; In-vitro; Inhibitors; Crystallization; Calcium; Oxalate; Synthetic urine; Juice.

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